Sr. Associate Scientist or Scientist, RNA Process Development - TechDigital
Seattle, WA
About the Job
Job Title Sr. Associate Scientist or Scientist, RNA Process Development
Location: Seattle, WA * 100% Onsite
Top Skills:
- Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
- Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
- Experience working with, storing, and analyzing purified nucleic acids and proteins
- Experience with optimizing molecular cloning reactions
- Experience with gene editing primary human cells or immortalized cell-lines
Seeking a highly motivated Sr. Associate Scientist, or Scientist, to work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing applications. As a part of the Gene Delivery and Editing Process Development (GDEPD) organization, the ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 200 nt).
A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop our next-generation gene edited therapies through the development of RNA oligomers. An outstanding candidate will be driven by a desire to create, optimize, and define processes with speed in a collaborative environment.
Key Responsibilities:
Design and execute nucleic acid ligation reactions using "splint ligation methodology
Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods
Perform purification of oligonucleotides using chromatography and/or preparative PAGE
Design and evaluate the performance of new biophysical assays to identify and assess CQA's
Manage samples and data related to gene editing platform process development
Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.)
Actively participate and support lab maintenance and continuous improvement initiatives
Maintain accurate and detailed laboratory notebook and documentation
Summarize and present data in group meetings and contribute to group discussions
Author technical reports, study memos, and protocols
Basic Qualifications:
STEM degree with 2+ years of relevant industry experience; title/compensation will be commensurate with education, experience, and skillset
Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
Experience working with, storing, and analyzing purified nucleic acids and proteins
Independently motivated with the ability to work in teams
Ability to communicate clearly and concisely through oral presentation and technical writing
Ability to leverage literature, internal and external resources to solve problems and develop new technologies
Possess a growth mindset and the ability to thrive in a fast-paced, highly collaborative environment
Preferred Qualifications:
Degree in biological or chemical engineering, biochemistry, or bio-related field
Experience with optimizing molecular cloning reactions
Experience with gene editing primary human cells or immortalized cell-lines
Experience with solid-phase oligonucleotide synthesis
Experience with bioinformatic tools used to predict RNA secondary structure
Experience with JMP for DoE design and analysis
Location: Seattle, WA * 100% Onsite
Top Skills:
- Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
- Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
- Experience working with, storing, and analyzing purified nucleic acids and proteins
- Experience with optimizing molecular cloning reactions
- Experience with gene editing primary human cells or immortalized cell-lines
Seeking a highly motivated Sr. Associate Scientist, or Scientist, to work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing applications. As a part of the Gene Delivery and Editing Process Development (GDEPD) organization, the ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 200 nt).
A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop our next-generation gene edited therapies through the development of RNA oligomers. An outstanding candidate will be driven by a desire to create, optimize, and define processes with speed in a collaborative environment.
Key Responsibilities:
Design and execute nucleic acid ligation reactions using "splint ligation methodology
Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods
Perform purification of oligonucleotides using chromatography and/or preparative PAGE
Design and evaluate the performance of new biophysical assays to identify and assess CQA's
Manage samples and data related to gene editing platform process development
Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.)
Actively participate and support lab maintenance and continuous improvement initiatives
Maintain accurate and detailed laboratory notebook and documentation
Summarize and present data in group meetings and contribute to group discussions
Author technical reports, study memos, and protocols
Basic Qualifications:
STEM degree with 2+ years of relevant industry experience; title/compensation will be commensurate with education, experience, and skillset
Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
Experience working with, storing, and analyzing purified nucleic acids and proteins
Independently motivated with the ability to work in teams
Ability to communicate clearly and concisely through oral presentation and technical writing
Ability to leverage literature, internal and external resources to solve problems and develop new technologies
Possess a growth mindset and the ability to thrive in a fast-paced, highly collaborative environment
Preferred Qualifications:
Degree in biological or chemical engineering, biochemistry, or bio-related field
Experience with optimizing molecular cloning reactions
Experience with gene editing primary human cells or immortalized cell-lines
Experience with solid-phase oligonucleotide synthesis
Experience with bioinformatic tools used to predict RNA secondary structure
Experience with JMP for DoE design and analysis
Source : TechDigital