We are seeking a highly motivated candidate to fill a full-time Senior Research Technician position in Dr. Kimberly Stegmaier’s laboratory at the Dana-Farber Cancer Institute. We are a translational pediatric oncology laboratory that uses a variety of genomic, biochemical, molecular, and cell biology approaches to identify new therapeutic targets, develop preclinical models of disease, and test novel therapeutics both in vitro and in vivo in pediatric cancers. The successful candidate will be a team player capable of handling multiple tasks and adaptable to a fast-paced research environment.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

• The senior technician will be responsible for conducting experiments to advance the research progress in the lab. As part of this work, they will be responsible for the following:

  -Working with mice and testing novel therapeutics in mouse models of leukemia and solid tumors. Previous experience working with mice is required.

  -Maintenance of cell lines and patient-derived xenograft (PDX) cells in culture, testing novel therapeutics in vivo and using in vitro proliferation assays.

  -Dosing and injection: Previous experience in dosing and injection by Oral, IV, Subcutaneous, and IP would be an asset but is not required.

  -Basic handling of DNA and RNA including sample isolation, PCR, and preparation of samples for sequencing.

  -Protein isolation from cells and primary murine samples, Western blotting, and immunoprecipitation experiments.

  -Transfection and retroviral/lentiviral transduction of murine and human cells for overexpression experiments or CRISPR-Cas9 knockout experiments.

  -Multi-color flow cytometry.

  -Preparation of reagents and media used in lab experiments.

  -Maintaining detailed records of their laboratory work, results, and data analyses.

  -Sharing responsibilities for operation and maintenance of standard lab equipment.

  The technician should be able to work independently, but also be open and receptive to suggestions and feedback from the team/project leader. Attention to detail in completing tasks and a strong work ethic is essential. This includes the willingness to critically read and keep up to date on the latest scientific literature, logically troubleshoot problems, offer both intellectual and technical input during collaborative meetings, and assist in the preparation of presentations, grants, and manuscripts. The technician should have flexibility in their schedule to work on some evenings and weekends as needed to meet project goals. We seek candidates who have at least 2 years of availability.

SUPERVISORY RESPONSIBILITIES:

No supervisory responsibilities.  Normally receives general instructions on routine work, detailed instructions on new projects or assignments.

• A bachelor’s or master’s degree in Biology, Biochemistry, Chemistry, or a related field is required.
• At least 1-2 years minimum of previous experience and familiarity with handling of mice is required.
• At least 2 years minimum of previous molecular biology laboratory research experience and familiarity with most of the techniques listed above required. 
• Excellent communication skills and ability to synthesize research findings for presentation in both oral and written formats.

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong.  As relentless as we are in our mission to reduce the burden of cancer for all, we are equally committed to diversifying our faculty and staff.  Cancer knows no boundaries and when it comes to hiring the most dedicated and diverse professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.

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