Senior Associate Scientist, Molecular Immunology - Johnson and Johnson
Cambridge, MA 02138
About the Job
At Janssen, we never stop working toward a future where disease is a thing of the past. We're the Pharmaceutical Companies of Johnson & Johnson, and you can count on us to keep working tirelessly to make that future a reality for patients everywhere, by fighting sickness with science, improving access with ingenuity, and healing hopelessness with heart. We focus on areas of medicine where we can make the biggest difference: Cardiovascular & Metabolism, Immunology, Infectious Diseases & Vaccines, Neuroscience, Oncology, and Pulmonary Arterial Hypertension. Visit us: www.janssen.com
As a member of the In Vivo Antibody Discovery Group, this role will be responsible for developing methods for the recovery of antibody V-genes from selected hybridoma cells or single B-cells using next generation sequencing (NGS) approaches. In-depth knowledge and extensive experience with BCR cloning using various methods including RT-PCR, Topo cloning, in-fusion cloning, multiplex NGS, and 10X Genomics are required. A strong understanding of B-cell recovery methods such as Hybridoma technology and primary B-cell sorting will also be important. This is a critical function in a multifaceted Biologics Discovery organization committed to bringing the next generation of biotherapeutic molecules to patients. We are looking for a motivated candidate with scientific and technical expertise who can collaborate across discovery areas to bring value to our portfolio of innovative drug concepts. Proficiency to work within a highly coordinated, fast-paced environment will be a significant attribute. An additional knowledge base in the areas of oncology, immunology, infectious disease, or cardiovascular disease would be a plus. If this sounds like you, come join our team!
Responsibilities :
• Antibody sequence recovery from selected hybridoma cells or B cells using NGS or 10X genomics
• NGS library preparation and sequencing and sequence analysis
• Antibody expression vector construction and DNA scale up for expression
• Small-scale (1 ml) antibody expression and purification
• Antibody characterization with Octet, ELISA, MSD and flow cytometry
• Regular presentation of data in team meeting, effective communication and coordination of work in highly matrixed project team environment Requirements
• Bachelor degree or Master degree in molecular biology, biochemistry, biophysics, immunology
• At least 2 years of relevant experience in the field of antibody discovery
• Working knowledge on antibody discovery using different approaches including hybridoma, B cell sorting
Preferred
• Hands-on experience in molecular cloning including V-gene primer design, in-fusion cloning, multiplex NGS, 10x Genomics
• In-depth understanding of antibody sequence analysis including germline analysis and CDR clustering and immune repertoire analysis and familiar with computation tools needed for antibody discovery workflow
• Antibody expression and purification
• Antibody characterization with Octet, ELISA, MSD and flow cytometry
• Hands-on experience on primary immune cell isolation from immunized animals
• Animal handling experience including immunization, blood collection and tissue collection
For more information on how we support the whole health of our employees throughout their wellness, career and life journey, please visit www.careers.jnj.com .
Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability
Eligible for benefits to include medical, dental, vision and time off as well as any others as provided for in the applicable Collective Bargaining Agreement
For additional general information on company benefits, please go to: - https://www.careers.jnj.com/employee-benefits