Scientist II from Collabera

What are the top 3-5 skills, experience, or education required for this position:

• Hands-on single-cell sequencing experience


• Hands-on spatial transcriptomics experience


• NGS data QC experience


• Hands-on NGS experience


• Molecular biology/NGS technical knowledge

Key Responsibilities:

• Support single-cell and spatial transcriptomic workflows. Perform sample and library prep, sequencing, initial data QC, and first-pass analysis of results. Closely work with NGS technical leads and partner with bioinformatics specialists and disease area scientists to interpret results and troubleshoot.


• Operate and maintain NGS and its supportive platforms within a centralized technology group. Prepare written summaries of experimental results and verbally present them at team meetings.


• Maintain accurate documentation of experiments, protocols, and results. Support inventory and maintenance.


• Effectively communicate with stakeholders, especially around experimental design, troubleshooting, responding to changes in priorities, and adjusting timelines.

Qualifications:

• Bachelor’s Degree or equivalent education and typically 7 years of experience, Master’s Degree or equivalent education and typically 5 years of experience, or PhD with 0-2 years experience in molecular biology, genetics, genomics, or a related field


• Demonstrated record of high proficiency, hands-on molecular biology experience with PCR-based assays, and technical knowledge of high-throughput genomic profiling technologies with emphasis on sequencing platforms and complex library preparation workflows for bulk RNA-seq, 10X Genomics scRNA-seq, and 10X Genomics Visium spatial transcriptomics


• Knowledge of QC metrics, interpretation, and demonstrated the use of approaches for assessing the quality of samples and libraries for bulkRNA, scRNA, and spatial profiling, such as capillary electrophoresis, qPCR, fluorometry, spectrophotometry, cell counting, and viability


• Proficiency with basic bioinformatics tools and sequencing data analysis concepts, such as SAV, bcl2fastq/BCLConvert, fastQC, and working in a UNIX HPC environment. Experience with 10X Genomics Cellranger and/or Spaceranger software is highly preferred.


• Experience being part of a centralized high-throughput genomic lab is highly desirable. Industry experience is preferred but willing to consider individuals with equivalent years of experience in academic or non-industry research core lab settings.


• High attention to detail, self-motivated, critical thinking, strong written and verbal communication skills


• Ability to work in a fast-paced, dynamic environment both collaboratively and independently


• Proven direct end-to-end hands-on experience with 10X Genomics technologies such as single-cell RNAseq or Visium is highly preferred.