Biologist Scientist - Veear Projects Inc

Deliverables:

• Work products and documentation to develop molecular cloning and cell differentiation protocols. - Ad-Hoc
• Work products and documents related to developing Client tools for CRISPR interference tool development in iPSCs - Ad-Hoc
• Work products and documents related to characterizing genetic pathways for Alzheimer's disease and related dementia (ADRD) using human cell-based models; use IPSC lines to develop the differentiation protocols - Ad-Hoc
• Work products and documents related to evaluating the stability of differentiated cells across clines and passages to identify cell lines with desirable characteristics for subsequent cell engineering. - Ad-Hoc
• Work products and documents related to providing insight into the effects of ADRD-associated mutations on molecular readouts in cell culture; conduct independent research relating to the genetics of ADRD. - Ad-Hoc
• Work products and documents related to applying published protocols for cell differentiation to IPSC lines, microscopy, and molecular analysis - Ad-Hoc
• Work products and documents related to updating and maintaining laboratory notebooks and databases; collect, analyze, and interpret data; prepare summaries and reports based on results and findings; draft and revise manuscripts for publication; attend meetings and present results and findings for staff discussion - Ad-Hoc
• Work products and documentation resulting from cell culture phenotyping of iPSC lines. - Ad- Hoc
• Work products and documents related to independent plasmid design, engineering, protocol execution, and validation of Client CRISPR tools - Ad-Hoc

Work Details:

• Independently design, troubleshoot, and carry out various molecular cloning experiments related to CRISPR nterference tool development and iPSC differentiation.
• Use SnapGene software to design and plan Client cloning projects.
• Work with staff on performing large-scale iPSC cell culture experiments.
• Work with staff on performing various experiments and procedures related to differentiation and phenotyping of induced pluripotent stem cell lines
• Work independently in designing, developing, and performing new methods.
• Provides technical experience needed to assist in studying the basic principles of plant and animal life
• Work with staff to characterize genetic pathways for Alzheimer's disease and related dementia (ADRD) using human cell-based models
• Evaluate the stability of iPSC and iPSC-derived cells across multiple engineered and non-engineered lines.
• Work with staff to apply published protocols for cell differentiation to IPSC lines, microscopy, and molecular analysis.
• Work with staff to provide insight into the effects of ADRD-associated mutations on molecular readouts in cell culture.
• Support other research projects.
• Collect, Analyze, interpret, and organize data and present results in team and departmental meetings.
• Keep detailed, organized, and accurate digital and physical experimental records.
• Update and maintain laboratory notebooks and databases.
• Attend meetings and present results and findings for staff discussion.
• Assists with performing basic laboratory experiments and procedures
• Transform bacteria with agar and liquid culture.
• Perform plasmid miniprep, maxiprep, gigaprep using column-based extraction kits.
• Prepare plasmid samples for sequencing by external companies.
• Perform genomic DNA extraction using column-based extraction kits.
• Prepare custom genomic DNA libraries for next-generation sequencing.
• Collaborate with other team members to carry out large-scale cell culture protocols using manual and high throughput robotic systems.
• Transduce iPSCs with lentivirus and transfect iPSCs with plasmids as part of routine cell culture experiments.
• Perform standard immunological techniques, including western blotting and immunostaining.
• Perform flow cytometry on iPSCs
• Provide scientific and technical expertise to all visiting fellows, post-docs, and technicians internal and external.
• Mentor junior level scientists by supporting other research projects.
• Prepares, organizes and maintains various lab samples, supplies, and equipment
• Train staff on the proper usage of laboratory instruments and equipment.
• Calibrate, maintain, and troubleshoot instruments and equipment in the laboratory.
• Maintain and perform staff training on scientific instrumentation, cell culture microscopies, microanalytical balances, centrifuges, automated platforms.
• Operate, troubleshoot, and train others to use laboratory equipment and software.
• Provide assistance to laboratory staff in designing experiments, using instrumentation, data acquisition and analysis, and preparing figures for publication.
• Review scientific equipment and resources including reagents, stocks of frozen cells, mammalian samples including acquisition, documentation, and storage.
• Mentor junior level scientists by training them on the proper use of equipment.
• Learns new laboratory techniques and procedures
• Develop new differentiation protocols for iPSC lines to differentiate into disease-relevant cell types like neurons, astrocytes, and microglia.
• Develop image analysis routines to analyze the effects of disease-associated mutations on cellular phenotypes.
• Willing to learn how to operate, and implement Client methods on liquid handling platforms (Hamilton, Biotek
• Willing to learn and maintain in-house flow cytometry machine

Minimum Education

• Master's

• Master's degree in cell biology, molecular biology, genetics, biochemistry, or a closely related discipline.

• Biology
• Neuroscience
• Molecular Biology

• MS Office Suite
• SnapGene