Associate Scientist - Pioneer Data Systems
Cambridge, MA 02139
About the Job
Position Details:
Our client, a world-leading Pharmaceutical Company in Cambridge, MA is currently looking for a Associate Scientist I to join their expanding team.
Job Title: Associate Scientist I (Cell Culture, DNA/RNA, Protein Purification)
Duration: 18 months contract, extendable up to 36 months
Location: Cambridge, MA
Note:
The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.
You may participate in the company group medical insurance plan
Job Description:
Associate Scientist
Summary:
Client's BioMedicine Design (BMD) is seeking a highly motivated biochemist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA.
Qualified candidate will play a unique and essential role in both RNA production and transient protein production research laboratories.
The groups support non-GMP RNA and transient protein lead production across Client's diverse biotherapeutic discovery portfolio to enable Pharmaceutical's research units and their pre-clinical therapeutic in-vitro and in-vivo needs.
The BEP production groups are looking for a laboratory technician to provide DNA, RNA and protein production support for the RNA and protein activities in BEP.
The candidate is expected to have a background in biochemistry techniques including nucleic acids, protein purification, and cell culture.
The successful candidate will grow bacterial and transient cultures, perform large-scale DNA production activities, synthesize RNA, and purify protein following standardized protocols.
The candidate will be a key contributor to the scientific team responsible for RNA and protein production and will report to the head of RNA Production.
Responsibilities:
Conduct routine end-to-end molecular biology and biochemistry techniques including but not limited to:
Cell transformations, day cultures, bacterial cultures, plasmid purification and digestions
DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS)
In vitro Transcription (IVT) and RNA characterization by gel electrophoresis, nanodrop and endotoxin testing
Transient cell culture techniques such as transfection, cell number and viability monitoring, cell lysis and protein harvest
Purification of proteins using liquid chromatography techniques
Protein characterization by analytical SEC, gel electrophoresis, nanodrop and endotoxin testing.
Maintaining daily work records in an organized electronic lab notebook. The candidate should also be proficient in using Microsoft products and an online database for request management
Maintaining reagent, DNA, RNA and protein inventory
Effectively communicating with team members to meet strict schedules and deadlines
Qualifications:
B.Sc in biochemistry, chemistry, biological/life sciences OR;
Associate's degree with a Certificate in Biotechnology OR;
High School diploma with at least 2+ years of relevant laboratory research school
Proficiency in biotechnology laboratory techniques and performing quantitative analysis
Experience working with bacterial cell lines and cell culture
Knowledge of nucleic acid purification and synthesis
Knowledge of protein purification techniques
Must have good practical skills in a laboratory setting such as keeping an organized laboratory notebook and maintaining laboratory cleanliness to minimize contamination
Ability to multitask and keep an organized record of daily work in an electronic lab notebook
Must be able to follow detailed protocols and weekly schedules
Must have the ability to concentrate while doing repetitive tasks
Must follow laboratory safety rules
Experience working with bacterial cell lines and cell culture preferred
In vitro Transcription (IVT) and RNA characterization by gel electrophoresis, nanodrop and endotoxin testing preferred
Protein characterization by analytical SEC, gel electrophoresis, nanodrop and endotoxin testing preferred
Location:
This is a fully onsite role in Cambridge, MA
Our client, a world-leading Pharmaceutical Company in Cambridge, MA is currently looking for a Associate Scientist I to join their expanding team.
Job Title: Associate Scientist I (Cell Culture, DNA/RNA, Protein Purification)
Duration: 18 months contract, extendable up to 36 months
Location: Cambridge, MA
Note:
The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.
You may participate in the company group medical insurance plan
Job Description:
Associate Scientist
Summary:
Client's BioMedicine Design (BMD) is seeking a highly motivated biochemist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA.
Qualified candidate will play a unique and essential role in both RNA production and transient protein production research laboratories.
The groups support non-GMP RNA and transient protein lead production across Client's diverse biotherapeutic discovery portfolio to enable Pharmaceutical's research units and their pre-clinical therapeutic in-vitro and in-vivo needs.
The BEP production groups are looking for a laboratory technician to provide DNA, RNA and protein production support for the RNA and protein activities in BEP.
The candidate is expected to have a background in biochemistry techniques including nucleic acids, protein purification, and cell culture.
The successful candidate will grow bacterial and transient cultures, perform large-scale DNA production activities, synthesize RNA, and purify protein following standardized protocols.
The candidate will be a key contributor to the scientific team responsible for RNA and protein production and will report to the head of RNA Production.
Responsibilities:
Conduct routine end-to-end molecular biology and biochemistry techniques including but not limited to:
Cell transformations, day cultures, bacterial cultures, plasmid purification and digestions
DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS)
In vitro Transcription (IVT) and RNA characterization by gel electrophoresis, nanodrop and endotoxin testing
Transient cell culture techniques such as transfection, cell number and viability monitoring, cell lysis and protein harvest
Purification of proteins using liquid chromatography techniques
Protein characterization by analytical SEC, gel electrophoresis, nanodrop and endotoxin testing.
Maintaining daily work records in an organized electronic lab notebook. The candidate should also be proficient in using Microsoft products and an online database for request management
Maintaining reagent, DNA, RNA and protein inventory
Effectively communicating with team members to meet strict schedules and deadlines
Qualifications:
B.Sc in biochemistry, chemistry, biological/life sciences OR;
Associate's degree with a Certificate in Biotechnology OR;
High School diploma with at least 2+ years of relevant laboratory research school
Proficiency in biotechnology laboratory techniques and performing quantitative analysis
Experience working with bacterial cell lines and cell culture
Knowledge of nucleic acid purification and synthesis
Knowledge of protein purification techniques
Must have good practical skills in a laboratory setting such as keeping an organized laboratory notebook and maintaining laboratory cleanliness to minimize contamination
Ability to multitask and keep an organized record of daily work in an electronic lab notebook
Must be able to follow detailed protocols and weekly schedules
Must have the ability to concentrate while doing repetitive tasks
Must follow laboratory safety rules
Experience working with bacterial cell lines and cell culture preferred
In vitro Transcription (IVT) and RNA characterization by gel electrophoresis, nanodrop and endotoxin testing preferred
Protein characterization by analytical SEC, gel electrophoresis, nanodrop and endotoxin testing preferred
Location:
This is a fully onsite role in Cambridge, MA
Source : Pioneer Data Systems